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Fast beneficial systemic anti-inflammatory effects of inhaled budesonide and formoterol on circulating lymphocytes in asthma

Rudiger, J. J. and Gencay, M. and Yang, J. Q. and Bihl, M. and Tamm, M. and Roth, M.. (2013) Fast beneficial systemic anti-inflammatory effects of inhaled budesonide and formoterol on circulating lymphocytes in asthma. Respirology, Vol. 18, H. 5. pp. 840-847.

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Official URL: http://edoc.unibas.ch/dok/A6338070

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Abstract

BACKGROUND AND OBJECTIVE: Inhaled glucocorticoids and long acting beta2 -agonists reduce airway inflammation. It is unclear if this effect is based on the local action of the drugs or is due to a systemic effect on circulating peripheral blood lymphocytes. We assessed whether inhaled budesonide and/or formoterol modify the activity of circulating peripheral blood lymphocytes. METHODS: Placebo controlled crossover design, including healthy (n = 10) or mild asthmatic males (n = 8). Blood was collected in the morning at 08:00 before drug inhalation, and drugs (placebo, budesonide 400 mug, formoterol 12 mug) were inhaled alone or in combination at 08:30. Four more blood samples were collected after inhalation at 09:00, 09:30, 12:30 and at 09:30 am on the following day. The activity of the glucocorticoid receptor, NFkappaB and IkappaB was determined in isolated lymphocytes. Lymphocytes were stimulated with lipopolysaccharide (LPS 10 mug/mL) for 24 h and interleukin (IL)-1beta, IL-6, IL-8, tumor necrosis factor (TNF)-alpha, eotaxin level were determined. Lymphocyte proliferation was induced by phytohaemagglutinin (PHA 10 mug/mL) over 24 h. RESULTS: When combined, the drugs synergistically activated the glucocorticoid receptor within 30 min but did not modify NFkappaB or IkappaB activity. Inhaled budesonide significantly reduced LPS-induced IL-1beta, IL-6, IL-8 and TNF-alpha secretion, while inhaled formoterol had no such effect; however when combined, the inhibitory effect of budesonide was significantly increased by formoterol. PHA-induced proliferation was reduced by both drugs alone and in combination. CONCLUSIONS: Combined budesonide and formoterol may reduce airway inflammation and immune reactivity of circulating lymphocytes through its local and systemic effects.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Pulmonary Cell Research (Roth/Tamm)
UniBasel Contributors:Tamm, Michael and Roth-Chiarello, Michael
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Blackwell
ISSN:1323-7799
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:06 Mar 2015 07:44
Deposited On:06 Mar 2015 07:44

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