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Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs

Okazaki, Y. and Furuno, M. and Kasukawa, T. and Adachi, J. and Bono, H. and Kondo, S. and Nikaido, I. and Osato, N. and Saito, R. and Suzuki, H. and Yamanaka, I. and Kiyosawa, H. and Yagi, K. and Tomaru, Y. and Hasegawa, Y. and Nogami, A. and Schonbach, C. and Gojobori, T. and Baldarelli, R. and Hill, D. P. and Bult, C. and Hume, D. A. and Quackenbush, J. and Schriml, L. M. and Kanapin, A. and Matsuda, H. and Batalov, S. and Beisel, K. W. and Blake, J. A. and Bradt, D. and Brusic, V. and Chothia, C. and Corbani, L. E. and Cousins, S. and Dalla, E. and Dragani, T. A. and Fletcher, C. F. and Forrest, A. and Frazer, K. S. and Gaasterland, T. and Gariboldi, M. and Gissi, C. and Godzik, A. and Gough, J. and Grimmond, S. and Gustincich, S. and Hirokawa, N. and Jackson, I. J. and Jarvis, E. D. and Kanai, A. and Kawaji, H. and Kawasawa, Y. and Kedzierski, R. M. and King, B. L. and Konagaya, A. and Kurochkin, I. V. and Lee, Y. and Lenhard, B. and Lyons, P. A. and Maglott, D. R. and Maltais, L. and Marchionni, L. and McKenzie, L. and Miki, H. and Nagashima, T. and Numata, K. and Okido, T. and Pavan, W. J. and Pertea, G. and Pesole, G. and Petrovsky, N. and Pillai, R. and Pontius, J. U. and Qi, D. and Ramachandran, S. and Ravasi, T. and Reed, J. C. and Reed, D. J. and Reid, J. and Ring, B. Z. and Ringwald, M. and Sandelin, A. and Schneider, C. and Semple, C. A. M. and Setou, M. and Shimada, K. and Sultana, R. and Takenaka, Y. and Taylor, M. S. and Teasdale, R. D. and Tomita, M. and Verardo, R. and Wagner, L. and Wahlestedt, C. and Wang, Y. and Watanabe, Y. and Wells, C. and Wilming, L. G. and Wynshaw-Boris, A. and Yanagisawa, M. and Yang, I. and Yang, L. and Yuan, Z. and Zavolan, M. and Zhu, Y. and Zimmer, A. and Carninci, P. and Hayatsu, N. and Hirozane-Kishikawa, T. and Konno, H. and Nakamura, M. and Sakazume, N. and Sato, K. and Shiraki, T. and Waki, K. and Kawai, J. and Aizawa, K. and Arakawa, T. and Fukuda, S. and Hara, A. and Hashizume, W. and Imotani, K. and Ishii, Y. and Itoh, M. and Kagawa, I. and Miyazaki, A. and Sakai, K. and Sasaki, D. and Shibata, K. and Shinagawa, A. and Yasunishi, A. and Yoshino, M. and Waterston, R. and Lander, E. S. and Rogers, J. and Birney, E. and Hayashizaki, Y.. (2002) Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature, Vol. 420 No. 6915. pp. 563-573.

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Official URL: http://edoc.unibas.ch/dok/A5259678

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Abstract

Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60,770 full-length mouse complementary DNA sequences. These are clustered into 33,409 'transcriptional units', contributing 90.1% of a newly established mouse transcriptome database. Of these transcriptional units, 4,258 are new protein-coding and 11,665 are new non-coding messages, indicating that non-coding RNA is a major component of the transcriptome. 41% of all transcriptional units showed evidence of alternative splicing. In protein-coding transcripts, 79% of splice variations altered the protein product. Whole-transcriptome analyses resulted in the identification of 2,431 sense-antisense pairs. The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Bioinformatics (Zavolan)
UniBasel Contributors:Zavolan, Mihaela
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Macmillan
ISSN:0028-0836
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:21
Deposited On:22 Mar 2012 13:23

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