edoc

Cryo-electron tomography provides novel insights into nuclear pore architecture : implications for nucleocytoplasmic transport

Stoffler, D. and Feja, B. and Fahrenkrog, B. and Walz, J. and Typke, D. and Aebi, U.. (2003) Cryo-electron tomography provides novel insights into nuclear pore architecture : implications for nucleocytoplasmic transport. Journal of molecular biology, Vol. 328, H. 1. pp. 119-130.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5259609

Downloads: Statistics Overview

Abstract

To go beyond the current structural consensus model of the nuclear pore complex (NPC), we performed cryo-electron tomography of fully native NPCs from Xenopus oocyte nuclear envelopes (NEs). The cytoplasmic face of the NPC revealed distinct anchoring sites for the cytoplasmic filaments, whereas the nuclear face was topped with a massive distal ring positioned above the central pore with indications of the anchoring sites for the nuclear basket filaments and putative intranuclear filaments. The rather "spongy" central framework of the NPC was perforated by an elaborate channel and void system, and at the membrane pore interface it exhibited distinct "handles" protruding into the lumen of the NE. The most variable structural moiety of the NPC was a rather tenuous central plug partially obstructing the central pore. Its mobile character was documented by time-lapse atomic force microscopy. Taken together, the new insights we gained into NPC structure support the notion that the NPC acts as a constrained diffusion pore for molecules and particles without retention signal and as an affinity gate for signal-bearing cargoes.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Fahrenkrog)
UniBasel Contributors:Fahrenkrog, Birthe
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0022-2836
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:21
Deposited On:22 Mar 2012 13:23

Repository Staff Only: item control page