A comprehensive analysis of the binding of anti-KIR antibodies to activating KIRs

Czaja, K. and Borer, A.-S. and Schmied, L. and Terszowski, G. and Stern, M. and Gonzalez, A.. (2014) A comprehensive analysis of the binding of anti-KIR antibodies to activating KIRs. Genes and immunity, Vol. 15, H. 1. pp. 33-37.

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Official URL: http://edoc.unibas.ch/dok/A6338090

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Analysis of killer cell immunoglobulin-like receptor (KIR) expression has been notoriously difficult because of the cross-reactivity of available antibodies, in particular between activating and inhibitory isoforms. We undertook a comprehensive study of available anti-KIR antibodies binding to activating KIRs (a-KIRs). Using cell lines stably transfected with a-KIRs (KIR2DS1-S5 and KIR3DS1), we confirmed documented binding specificities. In addition, we show that clones HPMA4 and 143211-previously assumed to be specific for KIR2DS1/L1 and KIR2DL1, respectively-bind KIR2DS5 and KIR2DS3 (HPMA4), and KIR2DS5 (143211). Other antibodies with previously undocumented binding were JJC11.6 (recognizing KIR2DS3) and 5.133 (recognizing all a-KIRs except KIR2DS1 and KIR2DS3). The novel KIR2DS5 reactivities were confirmed by blocking with soluble KIR-Fc fusion proteins, and by reverse transcriptase-PCR analysis of sorted primary natural killer cells. In conclusion, we show formerly undocumented binding properties of anti-KIR antibodies. These cross-reactivities should be taken into account when analyzing KIR expression.Genes and Immunity advance online publication, 31 October 2013; doi:10.1038/gene.2013.58.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Immunotherapy (Stern)
UniBasel Contributors:Stern, Martin Andreas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publ. Group.
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:04 Sep 2015 14:32
Deposited On:06 Feb 2015 09:59

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