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Formin-mediated actin polymerization at endothelial junctions is required for vessel lumen formation and stabilization

Phng, Li-Kun and Gebala, Véronique and Bentley, Katie and Philippides, Andrew and Wacker, Andrin and Mathivet, Thomas and Sauteur, Loïc and Stanchi, Fabio and Belting, Heinz-Georg and Affolter, Markus and Gerhardt, Holger. (2015) Formin-mediated actin polymerization at endothelial junctions is required for vessel lumen formation and stabilization. Developmental cell, Vol. 32, H. 1. pp. 123-132.

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Official URL: http://edoc.unibas.ch/dok/A6337885

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Abstract

During blood vessel formation, endothelial cells (ECs) establish cell-cell junctions and rearrange to form multicellular tubes. Here, we show that during lumen formation, the actin nucleator and elongation factor, formin-like 3 (fmnl3), localizes to EC junctions, where filamentous actin (F-actin) cables assemble. Fluorescent actin reporters and fluorescence recovery after photobleaching experiments in zebrafish embryos identified a pool of dynamic F-actin with high turnover at EC junctions in vessels. Knockdown of fmnl3 expression, chemical inhibition of formin function, and expression of dominant-negative fmnl3 revealed that formin activity maintains a stable F-actin content at EC junctions by continual polymerization of F-actin cables. Reduced actin polymerization leads to destabilized endothelial junctions and consequently to failure in blood vessel lumenization and lumen instability. Our findings highlight the importance of formin activity in blood vessel morphogenesis.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell Biology (Affolter)
UniBasel Contributors:Affolter, Markus
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Cell Press
ISSN:1534-5807
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:06 Feb 2015 09:59
Deposited On:06 Feb 2015 09:59

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