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Cellular Trojan Horse Based Polymer Nanoreactors with Light-Sensitive Activity

Baumann, Patric and Spulber, Mariana and Dinu, Ionel Adrian and Palivan, Cornelia G.. (2014) Cellular Trojan Horse Based Polymer Nanoreactors with Light-Sensitive Activity. Journal of Physical Chemistry B, 118 (31). pp. 9361-9370.

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Official URL: http://edoc.unibas.ch/dok/A6329082

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Abstract

Stimulus-sensitive systems at the nanoscale represent ideal candidates for improving therapeutic and diagnostic approaches by producing rapid responses to the presence of specific molecules or conditions either by changing properties or by acting “on demand”. Here we introduce an optimized light-sensitive nanoreactor based on encapsulation of a photosensitizer inside polymer vesicles to serve as an efficient source of reactive oxygen species (ROS) “on demand”. Two types of amphiphilic block copolymers, poly(2-methyloxazoline)-block-poly(dimethylsiloxane)-block-poly(2-methyloxazoline), PMOXA–PDMS–PMOXA, and poly(N-vinylpyrrolidone)-block-poly(dimethylsiloxane)-block-poly(N-vinylpyrrolidone), PNVP–PDMS–PNVP, were used to encapsulate Rose Bengal–bovine serum albumin (RB–BSA) inside the cavity of vesicles. The difference of copolymers molecular properties (hydrophobic to hydrophilic ratio, different chemical nature of the hydrophilic block) influenced the encapsulation ability, and uptake by cells, allowing therefore a selection of the most efficient polymer system. Nanoreactors were optimized in terms of (i) size, (ii) stability, and (iii) encapsulation efficiency based on a combination of light scattering, TEM, and UV–vis spectroscopy. By illumination, encapsulated RB–BSA conjugates generated in situ ROS, which diffused through the polymer membrane to the environment of the vesicles, as proved by electron spin resonance spectroscopy (ESR). Optimum illumination conditions were obtained based on the effect of the illumination time on the amount of ROS produced in situ by the encapsulated RB–BSA conjugates. ROS diffusion monitored by ESR was dependent on the molecular weight of copolymer that influences the thickness of the polymer membrane. Upon uptake into HeLa cells our nontoxic nanoreactors acted as a Trojan horse: they produced illumination-controlled ROS in sufficient amounts to induce cell death under photodynamic therapy (PDT) conditions. Straightforward production, stability, and Trojan horse activity inside cells support our light-sensitive nanoreactors for medical applications which require ROS to be generated with precise time and space control.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Palivan, Cornelia G
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
ISSN:1520-6106
e-ISSN:1520-5207
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:10 Apr 2017 07:00
Deposited On:06 Feb 2015 09:58

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