Braun, A. and Barde, Y. A. and Lottspeich, F. and Mewes, W. and Thoenen, H.. (1987) N-terminal sequence of pig brain choline acetyltransferase purified by a rapid procedure. Journal of Neurochemistry, Vol. 48, H. 1. pp. 16-21.
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Official URL: http://edoc.unibas.ch/dok/A5259571
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Abstract
A procedure is reported that allows the purification and amino terminal sequencing of pig brain choline acetyltransferase. The enzyme (present in extremely low amounts in this tissue) is eluted together with its antibody from an affinity column by a mild pH shift and the resulting enzyme-antibody complex separated by gel electrophoresis. The band corresponding to the enzyme is electroeluted from the gel using volatile solutions allowing the direct determination of the amino acid composition and partial sequence. The first 11 residues are: Pro-Ile-Leu-Glu-Lys-Thr-Pro-Pro-Lys-Met-Ala.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Pharmacology/Neurobiology (Barde) |
|---|---|
| UniBasel Contributors: | Barde, Yves-Alain |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Wiley-Blackwell |
| ISSN: | 0022-3042 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Last Modified: | 22 Mar 2012 14:21 |
| Deposited On: | 22 Mar 2012 13:22 |
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