edoc

Functional surface engineering by nucleotide-modulated potassium channel insertion into polymer membranes attached to solid supports

Kowal, Justyna Ł and Kowal, Julia K. and Wu, Dalin and Stahlberg, Henning and Palivan, Cornelia G. and Meier, Wolfgang P.. (2014) Functional surface engineering by nucleotide-modulated potassium channel insertion into polymer membranes attached to solid supports. Biomaterials, 35 (26). pp. 7286-7294.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A6329067

Downloads: Statistics Overview

Abstract

Planar solid-supported membranes based on amphiphilic block copolymers represent promising systems for the artificial creation of structural surfaces. Here we introduce a method for engineering functional planar solid-supported membranes through insertion of active biomolecules. We show that membranes based on poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PDMS-b-PMOXA) amphiphilic diblock copolymers, which mimic natural membranes, are suitable for hosting biomolecules. Our strategy allows preparation of large-area, well-ordered polymer bilayers via Langmuir-Blodgett and Langmuir-Schaefer transfers, and insertion of biomolecules by using Bio-Beads. We demonstrate that a model membrane protein, the potassium channel from the bacterium Mesorhizobium loti, remains functional after insertion into the planar solid-supported polymer membrane. This approach can be easily extended to generate a platform of functional solid-supported membranes by insertion of different hydrophobic biomolecules, and employing different types of solid substrates for desired applications.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P. and Stahlberg, Henning and Palivan, Cornelia G
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:0142-9612
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:05 Apr 2017 14:55
Deposited On:09 Jan 2015 09:25

Repository Staff Only: item control page