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In Planta Somatic Homologous Recombination Assay Revisited : a Successful and Versatile, but Delicate Tool

Puchta, H. and Hohn, B.. (2012) In Planta Somatic Homologous Recombination Assay Revisited : a Successful and Versatile, but Delicate Tool. The plant cell, Vol. 24, H. 11. pp. 4324-4331.

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Official URL: http://edoc.unibas.ch/dok/A6308446

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Abstract

Marker-transgene-dependent lines of Arabidopsis thaliana measuring somatic homologous recombination (SHR) have been available for almost two decades. Here we discuss mechanisms of marker-gene restoration, comment on results obtained using the reporter lines, and stress how caution must be applied to avoid experimental problems or false interpretation in the use of SHR reporter lines. Although theoretically possible, we conclude that explanations other than SHR are unlikely to account for restoration of marker gene expression in the SHR lines when used with appropriate controls. We provide an overview of some of the most important achievements obtained with the SHR lines, give our view of the limitations of the system, and supply the reader with suggestions on the proper handling of the SHR lines. We are convinced that SHR lines are and will remain in the near future a valuable tool to explore the mechanism and influence of external and internal factors on genome stability and DNA repair in plants.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Botanik > Pflanzenphysiologie Pathogenabwehr (Boller)
UniBasel Contributors:Hohn, Barbara
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Society of Plant Biologists
ISSN:1040-4651
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:07 Nov 2014 08:29
Deposited On:07 Nov 2014 08:29

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