edoc

Controlled human malaria infection of Tanzanians by intradermal injection of aseptic, purified, cryopreserved Plasmodium falciparum sporozoites

Shekalaghe, Seif and Rutaihwa, Mastidia and Billingsley, Peter F. and Chemba, Mwajuma and Daubenberger, Claudia A. and James, Eric R. and Mpina, Maximillian and Ali Juma, Omar and Schindler, Tobias and Huber, Eric and Gunasekera, Anusha and Manoj, Anita and Simon, Beatus and Saverino, Elizabeth and Church, L. W. Preston and Hermsen, Cornelus C. and Sauerwein, Robert W. and Plowe, Christopher and Venkatesan, Meera and Sasi, Philip and Lweno, Omar and Mutani, Paul and Hamad, Ali and Mohammed, Ali and Urassa, Alwisa and Mzee, Tutu and Padilla, Debbie and Ruben, Adam and Sim, B. Kim Lee and Tanner, Marcel and Abdulla, Salim and Hoffman, Stephen L.. (2014) Controlled human malaria infection of Tanzanians by intradermal injection of aseptic, purified, cryopreserved Plasmodium falciparum sporozoites. American journal of tropical medicine and hygiene, Vol. 91, H. 3. pp. 471-480.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A6308428

Downloads: Statistics Overview

Abstract

Controlled human malaria infection (CHMI) by mosquito bite has been used to assess anti-malaria interventions in < 1,500 volunteers since development of methods for infecting mosquitoes by feeding on Plasmodium falciparum (Pf) gametocyte cultures. Such CHMIs have never been used in Africa. Aseptic, purified, cryopreserved Pf sporozoites, PfSPZ Challenge, were used to infect Dutch volunteers by intradermal injection. We conducted a double-blind, placebo-controlled trial to assess safety and infectivity of PfSPZ Challenge in adult male Tanzanians. Volunteers were injected intradermally with 10,000 (N = 12) or 25,000 (N = 12) PfSPZ or normal saline (N = 6). PfSPZ Challenge was well tolerated and safe. Eleven of 12 and 10 of 11 subjects, who received 10,000 and 25,000 PfSPZ respectively, developed parasitemia. In 10,000 versus 25,000 PfSPZ groups geometric mean days from injection to Pf positivity by thick blood film was 15.4 versus 13.5 (P = 0.023). Alpha-thalassemia heterozygosity had no apparent effect on infectivity. PfSPZ Challenge was safe, well tolerated, and infectious.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Clinical Immunology (Daubenberger)
UniBasel Contributors:Daubenberger, Claudia and Tanner, Marcel
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Williams and Wilkins
ISSN:0002-9637
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:07 Nov 2014 08:28
Deposited On:07 Nov 2014 08:28

Repository Staff Only: item control page