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Phenotypic variation of salmonella in host tissues delays eradication by antimicrobial chemotherapy

Claudi, Beatrice and Spröte, Petra and Chirkova, Anna and Personnic, Nicolas and Zankl, Janine and Schürmann, Nura and Schmidt, Alexander and Bumann, Dirk. (2014) Phenotypic variation of salmonella in host tissues delays eradication by antimicrobial chemotherapy. Cell, 158 (4). pp. 722-733.

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Official URL: http://edoc.unibas.ch/dok/A6289112

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Abstract

Antibiotic therapy often fails to eliminate a fraction of transiently refractory bacteria, causing relapses and chronic infections. Multiple mechanisms can induce such persisters with high antimicrobial tolerance in vitro, but their in vivo relevance remains unclear. Using a fluorescent growth rate reporter, we detected extensive phenotypic variation of Salmonella in host tissues. This included slow-growing subsets as well as well-nourished fast-growing subsets driving disease progression. Monitoring of Salmonella growth and survival during chemotherapy revealed that antibiotic killing correlated with single-cell division rates. Nondividing Salmonella survived best but were rare, limiting their impact. Instead, most survivors originated from abundant moderately growing, partially tolerant Salmonella. These data demonstrate that host tissues diversify pathogen physiology, with major consequences for disease progression and control.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Bumann)
UniBasel Contributors:Bumann, Dirk and Claudi, Beatrice and Personnic, Nicolas and Zankl, Janine and Schürmann, Nura and Schmidt, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Cell Press
ISSN:0092-8674
e-ISSN:1097-4172
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:27 Nov 2017 07:47
Deposited On:10 Oct 2014 09:19

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