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Cloning and expression of cDNA for human vascular anticoagulant, a Ca2+-dependent phospholipid-binding protein

Maurer-Fogy, I. and Reutelingsperger, C. P. and Pieters, J. and Bodo, G. and Stratowa, C. and Hauptmann, R.. (1988) Cloning and expression of cDNA for human vascular anticoagulant, a Ca2+-dependent phospholipid-binding protein. European journal of biochemistry, Vol. 174, H. 4. pp. 585-592.

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Official URL: http://edoc.unibas.ch/dok/A5259440

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Abstract

Based on sequence information from tryptic peptides an almost full-size cDNA coding for the human vascular anticoagulant was isolated from a placental cDNA library and sequenced. The coding region was cloned into an Escherichia coli expression vector and the protein expressed at high levels. The recombinant protein was purified and found to be indistinguishable from its natural counterpart in several biological assays.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Biochemistry (Pieters)
UniBasel Contributors:Pieters, Jean
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Blackwell
ISSN:0014-2956
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:21
Deposited On:22 Mar 2012 13:22

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