Role of protein kinase G in growth and glutamine metabolism of Mycobacterium bovis BCG

Nguyen, L. and Walburger, A. and Houben, E. and Koul, A. and Muller, S. and Morbitzer, M. and Klebl, B. and Ferrari, G. and Pieters, J.. (2005) Role of protein kinase G in growth and glutamine metabolism of Mycobacterium bovis BCG. Journal of bacteriology, 187 (16). pp. 5852-5856.

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Official URL: http://edoc.unibas.ch/dok/A5259405

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The survival of pathogenic mycobacteria in macrophages requires the eukaryotic enzyme-like serine/threonine protein kinase G. This kinase with unknown specificity is secreted into the cytosol of infected macrophages and inhibits phagosome-lysosome fusion. The pknG gene is the terminal gene in a putative operon containing glnH, encoding a protein potentially involved in glutamine uptake. Here, we report that the deletion of pknG did not affect either glutamine uptake or intracellular glutamine concentrations. In vitro growth of Mycobacterium bovis BCG lacking pknG was identical to that of the wild type. We conclude that in M. bovis BCG, glutamine metabolism is not regulated by protein kinase G.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Biochemistry (Pieters)
UniBasel Contributors:Pieters, Jean
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Society for Microbiology
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:21 Sep 2017 12:54
Deposited On:22 Mar 2012 13:22

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