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Field comparison of circulating antibody assays versus circulating antigen assays for the detection of Schistosomiasis japonica in endemic areas of China

Cai, Yu-Chun and Xu, Jun-Fang and Steinmann, Peter and Chen, Shao-Hong and Chu, Yan-Hong and Tian, Li-Guang and Chen, Mu-Xin and Li, Hao and Lu, Yan and Zhang, Ling-Ling and Zhou, Yang and Chen, Jia-Xu. (2014) Field comparison of circulating antibody assays versus circulating antigen assays for the detection of Schistosomiasis japonica in endemic areas of China. Parasites and Vectors, 7 (138).

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Official URL: http://edoc.unibas.ch/dok/A6254458

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Abstract

Schistosomiasis remains a serious public health problem in affected countries, and routine, highly sensitive and cost-effective diagnostic methods are lacking. We evaluated two immunodiagnostic techniques for the detection of Schistosoma japonicum infections: circulating antibody and circulating antigen assays.; A total of 1864 individuals (between 6 and 72 years old) residing in five administrative villages in Hubei province were screened by serum examination with an indirect hemagglutination assay (IHA). The positive individuals (titer ≥20 in IHA) were reconfirmed by stool examination with the Kato-Katz method (three slides from a single stool specimen). Samples of good serum quality and a volume above 0.5 ml were selected for further testing with two immunodiagnostic antibody (DDIA and ELISA) and two antigen (ELISA) assays.; The average antibody positive rate in the five villages was 12.7%, while the average parasitological prevalence was 1.50%; 25 of the 28 egg-positive samples were also circulating antigen-positive. Significant differences were observed between the prevalence according to the Kato-Katz method and all three immunodiagnostic antibody assays (P-value >0.0001). Similar differences were observed between the Kato-Katz method and the two immunodiagnostic antigen assays (P-value >0.0001) and between the antigen and antibody assays (P-value >0.0001).; Both circulating antibody and circulating antigen assays had acceptable performance characteristics. Immunodiagnostic techniques to detect circulating antigens have potential to be deployed for schistosomiasis japonica screening in the endemic areas.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Swiss Centre for International Health > Health Systems Support (Prytherch)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
UniBasel Contributors:Steinmann, Peter
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:BioMed Central
ISSN:1756-3305
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:15 Nov 2016 15:57
Deposited On:15 Aug 2014 07:16

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