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Survival of pathogenic mycobacteria in macrophages is mediated through autophosphorylation of protein kinase G

Scherr, Nicole and Müller, Philipp and Perisa, Damir and Combaluzier, Benoit and Jenö, Paul and Pieters, Jean. (2009) Survival of pathogenic mycobacteria in macrophages is mediated through autophosphorylation of protein kinase G. Journal of Bacteriology, 191 (14). pp. 4546-4554.

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Official URL: http://edoc.unibas.ch/dok/A5259386

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Abstract

Pathogenic mycobacteria survive within macrophages through the inhibition of phagosome-lysosome fusion. A crucial factor for avoiding lysosomal degradation is the mycobacterial serine/threonine protein kinase G (PknG). PknG is released into the macrophage cytosol upon mycobacterial infection, suggesting that PknG might exert its activity by interfering with host signaling cascades, but the mode of action of PknG remains unknown. Here, we show that PknG undergoes autophosphorylation on threonine residues located at the N terminus. In contrast to all other mycobacterial kinases investigated thus far, autophosphorylation of PknG was not involved in the regulation of its kinase activity. However, autophosphorylation was crucial for the capacity of PknG to promote mycobacterial survival within macrophages. These results will contribute to a better understanding of the virulence mechanisms of pathogenic mycobacteria and may help to design improved inhibitors of PknG to be developed as antimycobacterial compounds.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Biochemistry (Pieters)
05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Mass Spectrometry (Jenö)
UniBasel Contributors:Pieters, Jean and Jenö, Paul and Moes, Suzanne
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Society for Microbiology
ISSN:0021-9193
e-ISSN:1098-5530
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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edoc DOI:
Last Modified:21 Sep 2020 14:29
Deposited On:22 Mar 2012 13:22

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