Selective transport control on molecular velcro made from intrinsically disordered proteins

Schleicher, Kai D. and Dettmer, Simon D. and Kapinos, Larisa E. and Pagliara, Stefano and Keyser, Ulrich F. and Jeney, Sylvia and Lim, Roderick Y. H.. (2014) Selective transport control on molecular velcro made from intrinsically disordered proteins. Nature nanotechnology, Vol. 9 H. 7 , S. 525–530.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A6271917

Downloads: Statistics Overview


The selectivity and speed of many biological transport processes transpire from a 'reduction of dimensionality' that confines diffusion to one or two dimensions instead of three. This behaviour remains highly sought after on polymeric surfaces as a means to expedite diffusional search processes in molecular engineered systems. Here, we have reconstituted the two-dimensional diffusion of colloidal particles on a molecular brush surface. The surface is composed of phenylalanine-glycine nucleoporins (FG Nups)--intrinsically disordered proteins that facilitate selective transport through nuclear pore complexes in eukaryotic cells. Local and ensemble-level experiments involving optical trapping using a photonic force microscope and particle tracking by video microscopy, respectively, reveal that 1-µm-sized colloidal particles bearing nuclear transport receptors called karyopherins can exhibit behaviour that varies from highly localized to unhindered two-dimensional diffusion. Particle diffusivity is controlled by varying the amount of free karyopherins in solution, which modulates the multivalency of Kap-binding sites within the molecular brush. We conclude that the FG Nups resemble stimuli-responsive molecular 'velcro', which can impart 'reduction of dimensionality' as a means of biomimetic transport control in artificial environments.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nanobiology Argovia (Lim)
UniBasel Contributors:Lim, Roderick Y.H. and Schleicher, Kai and Kapinos Schneider, Larisa E. E
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:05 Jun 2015 08:52
Deposited On:18 Jul 2014 09:10

Repository Staff Only: item control page