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Development of a simple microarray for genotyping HIV-1 drug resistance mutations in the reverse transcriptase gene in rural Tanzania

Masimba, Pax and Gare, Janet and Klimkait, Thomas and Tanner, Marcel and Felger, Ingrid. (2014) Development of a simple microarray for genotyping HIV-1 drug resistance mutations in the reverse transcriptase gene in rural Tanzania. Tropical medicine and international health, 19 (6). pp. 664-671.

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Official URL: http://edoc.unibas.ch/dok/A6263095

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Abstract

Aiming at a simple, inexpensive and robust tool for HIV-1 drug resistance genotyping during antiretroviral therapy (ART) we developed and validated a microarray-based detection of 25 drug resistance mutations most relevant for the Tanzanian ART regimen.; A reverse transcriptase gene fragment was reverse-transcribed and amplified by reverse transcription-polymerase chain reaction (RT-PCR). Primers for mini-sequencing were designed based on alignments of the most prevalent local HIV-1 variants. Tagged primers were extended by fluorochrome-labelled dideoxynuclotide triphosphate (ddNTPs) to indicate the single-nucleotide polymorphism (SNP) allele of the sample tested, followed by hybridisation on treated microarray slides. Images were analysed with a laser scanner and genotype calling was performed using in-house developed software.; The microarray was validated with four cloned HIV-1 genome fragments from a Swiss HIV-1 cohort and 102 HIV-1 sequences amplified from the Tanzanian target population (field samples). Results were concordant with the Sanger sequencing SNP profile in 92.7% of 2550 SNP data points compared. Lack of signals in small number of SNPs was due to either failure in the extension reaction or hybridisation owing to mismatches between PCR product and extension primer.; Our study demonstrates the feasibility of hybridisation-based genotyping of drug resistance mutations of HIV, even though our microarray, which was designed for population studies, achieved only correct assignment of 92% of all SNPs in the tested samples.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Former Units within Swiss TPH > Molecular Diagnostics (Felger)
UniBasel Contributors:Tanner, Marcel and Felger, Ingrid
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Blackwell Science
ISSN:1360-2276
Note:Publication type according to Uni Basel Research Database: Journal article -- This is the peer reviewed version of the following article: Masimba, Pax and Gare, Janet and Klimkait, Thomas and Tanner, Marcel and Felger, Ingrid. (2014) Development of a simple microarray for genotyping HIV-1 drug resistance mutations in the reverse transcriptase gene in rural Tanzania. Tropical medicine and international health, 19 (6). pp. 664-671 , which has been published in final form at [see DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Language:English
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Last Modified:18 Apr 2018 08:03
Deposited On:18 Jul 2014 07:42

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