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Mixed infection by Legionella pneumophila in outbreak patients

Coscollá, Mireia and Fernández, Carmen and Colomina, Javier and Sánchez-Busó, Leonor and González-Candelas, Fernando. (2014) Mixed infection by Legionella pneumophila in outbreak patients. Zentralblatt für Bakteriologie = International journal of medical microbiology, Vol. 304, H. 3/4. pp. 307-313.

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Official URL: http://edoc.unibas.ch/dok/A6263092

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Abstract

During the molecular epidemiological study of a legionellosis outbreak, we obtained sequence based typing (SBT) profiles from uncultured respiratory samples of 15 affected patients. We detected several distinct allelic profiles some of which were a mixture of alleles present in the more common profiles. Chromatograms from the sequences of one patient with mixed profile showed polymorphisms in several positions, which could result from the simultaneous presence of different Legionella variants in the sample. In order to test this possibility, we cloned PCR amplification products from six loci for two patients with a mixed profile and a patient with a pure profile. After obtaining around 20 sequences for each locus of three patients, we detected several variants in two of them and two variants in the third one. In summary, the three analyzed patients showed evidence of more than one Legionella variant during the acute infection. These results indicate that probably some patients were infected by more than one strain, which could be due to co-infection from the same environmental source or, alternatively, to independent infections in a very short period of time. Although our data cannot discriminate between these hypotheses, these results suggest that Legionella infection patterns can be more complex than previously assumed. None of the environmental samples analyzed during this outbreak was even similar to any of the clinical ones.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Tuberculosis Research (Gagneux)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
UniBasel Contributors:Coscollá, Mireja
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:G. Fischer
ISSN:0934-8840
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:18 Jul 2014 09:09
Deposited On:18 Jul 2014 07:42

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