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Structural basis for microtubule recognition by the human kinetochore Ska complex

Abad, Maria Alba and Medina, Bethan and Santamaria, Anna and Zou, Juan and Plasberg-Hill, Carla and Madhumalar, Arumugam and Jayachandran, Uma and Redli, Patrick Marc and Rappsilber, Juri and Nigg, Erich A. and Jeyaprakash, A. Arockia. (2014) Structural basis for microtubule recognition by the human kinetochore Ska complex. Nature communications, Vol. 5 , Article Nr. 2964.

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Official URL: http://edoc.unibas.ch/dok/A6223396

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Abstract

The ability of kinetochores (KTs) to maintain stable attachments to dynamic microtubule structures ('straight' during microtubule polymerization and 'curved' during microtubule depolymerization) is an essential requirement for accurate chromosome segregation. Here we show that the kinetochore-associated Ska complex interacts with tubulin monomers via the carboxy-terminal winged-helix domain of Ska1, providing the structural basis for the ability to bind both straight and curved microtubule structures. This contrasts with the Ndc80 complex, which binds straight microtubules by recognizing the dimeric interface of tubulin. The Ska1 microtubule-binding domain interacts with tubulins using multiple contact sites that allow the Ska complex to bind microtubules in multiple modes. Disrupting either the flexibility or the tubulin contact sites of the Ska1 microtubule-binding domain perturbs normal mitotic progression, explaining the critical role of the Ska complex in maintaining a firm grip on dynamic microtubules.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg)
UniBasel Contributors:Nigg, Erich A.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Nature Publishing Group
ISSN:2041-1723
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:27 Mar 2014 13:13
Deposited On:27 Mar 2014 13:13

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