pH-dependent coordination of Ag(I) ions by histidine : experiment, theory, and a model for SilE

Mirolo, Laurent and Schmidt, Tobias and Eckhardt, Sonja and Meuwly, Markus and Fromm, Katharina M.. (2013) pH-dependent coordination of Ag(I) ions by histidine : experiment, theory, and a model for SilE. Chemistry, 19 (5). pp. 1754-1761.

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Official URL: http://edoc.unibas.ch/dok/A6223433

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Artificial implants and biomaterials lack the natural defense system of our body and, thus, have to be protected from bacterial adhesion and biofilm formation. In addition to the increasing number of implanted objects, the resistance of bacteria is also an important problem. Silver ions are well-known for their antimicrobial properties, yet not a lot is known about their mode of action. Silver is expected to interact on many levels, thus the development of silver resistance is very difficult. Nevertheless, some bacteria are able to resist silver, even at higher concentrations. One such defense mechanism of bacteria against heavy-metal intoxication includes an efflux system. SilE, a periplasmic silver-binding protein that is involved in this defense mechanism, has been shown to possess numerous histidine functions, which strongly bind to silver atoms, as demonstrated by ourselves previously. Herein, we address the question of how histidine binds to silver ions as a function of pH value. This property is important because the local proton concentration in cells varies. Thus, we solved the crystal structures of histidine-silver complexes at different pH values and also investigated the influence of the amino-acid configuration. These results were completed by DFT calculations on the binding strength and packing effects and led to the development of a model for the mode of action of SilE.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Physikalische Chemie (Meuwly)
UniBasel Contributors:Meuwly, Markus
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:07 Dec 2016 12:26
Deposited On:27 Mar 2014 13:12

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