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The Bi-Functional Organization of Human Basement Membranes

Halfter, Willi and Monnier, Christophe and Mueller, David and Oertle, Philipp and Uechi, Guy and Balasubramani, Manimalha and Safi, Farhad and Lim, Roderick and Loparic, Marko and Henrich, Paul Bernhard. (2013) The Bi-Functional Organization of Human Basement Membranes. PLoS ONE, 8 (7). e67660.

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Official URL: http://edoc.unibas.ch/dok/A6223220

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Abstract

The current basement membrane (BM) model proposes a single-layered extracellular matrix (ECM) sheet that is predominantly composed of laminins, collagen IVs and proteoglycans. The present data show that BM proteins and their domains are asymmetrically organized providing human BMs with side-specific properties: A) isolated human BMs roll up in a side-specific pattern, with the epithelial side facing outward and the stromal side inward. The rolling is independent of the curvature of the tissue from which the BMs were isolated. B) The epithelial side of BMs is twice as stiff as the stromal side, and C) epithelial cells adhere to the epithelial side of BMs only. Side-selective cell adhesion was also confirmed for BMs from mice and from chick embryos. We propose that the bi-functional organization of BMs is an inherent property of BMs and helps build the basic tissue architecture of metazoans with alternating epithelial and connective tissue layers.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nanobiology Argovia (Lim)
UniBasel Contributors:Lim, Roderick Y.H.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Public Library of Science
e-ISSN:1932-6203
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:31 Aug 2018 06:39
Deposited On:27 Mar 2014 13:12

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