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Extensive mass spectrometry-based analysis of the fission yeast proteome : the Schizosaccharomyces pombe Peptide Atlas

Gunaratne, Jayantha and Schmidt, Alexander and Quandt, Andreas and Neo, Suat Peng and Saraç, Omer Sinan and Gracia, Tannia and Loguercio, Salvatore and Ahrné, Erik and Xia, Rachel Li Hai and Tan, Keng Hwa and Lössner, Christopher and Bähler, Jürg and Beyer, Andreas and Blackstock, Walter and Aebersold, Ruedi. (2013) Extensive mass spectrometry-based analysis of the fission yeast proteome : the Schizosaccharomyces pombe Peptide Atlas. Molecular & cellular proteomics, Vol. 12, H. 6. pp. 1741-1751.

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Official URL: http://edoc.unibas.ch/dok/A6211851

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Abstract

We report a high quality and system-wide proteome catalogue covering 71% (3,542 proteins) of the predicted genes of fission yeast, Schizosaccharomyces pombe, presenting the largest protein dataset to date for this important model organism. We obtained this high proteome and peptide (11.4 peptides/protein) coverage by a combination of extensive sample fractionation, high resolution Orbitrap mass spectrometry, and combined database searching using the iProphet software as part of the Trans-Proteomics Pipeline. All raw and processed data are made accessible in the S. pombe PeptideAtlas. The identified proteins showed no biases in functional properties and allowed global estimation of protein abundances. The high coverage of the PeptideAtlas allowed correlation with transcriptomic data in a system-wide manner indicating that post-transcriptional processes control the levels of at least half of all identified proteins. Interestingly, the correlation was not equally tight for all functional categories ranging from r(s) <0.80 for proteins involved in translation to r(s) >0.45 for signal transduction proteins. Moreover, many proteins involved in DNA damage repair could not be detected in the PeptideAtlas despite their high mRNA levels, strengthening the translation-on-demand hypothesis for members of this protein class. In summary, the extensive and publicly available S. pombe PeptideAtlas together with the generated proteotypic peptide spectral library will be a useful resource for future targeted, in-depth, and quantitative proteomic studies on this microorganism.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Proteomics (Schmidt)
UniBasel Contributors:Schmidt, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:The American Society for Biochemistry and Molecular Biology
ISSN:1535-9484
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:31 Jan 2014 09:51
Deposited On:31 Jan 2014 09:51

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