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Study on the interrelated effects of capillary diameter, background electrolyte concentration, and flow rate in pressure assisted capillary electrophoresis with contactless conductivity detection

Mai, T. D. and Hauser, P. C.. (2013) Study on the interrelated effects of capillary diameter, background electrolyte concentration, and flow rate in pressure assisted capillary electrophoresis with contactless conductivity detection. Electrophoresis, Vol. 34, H. 12. pp. 1796-1803.

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Official URL: http://edoc.unibas.ch/dok/A6212109

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Abstract

A detailed study on the effect of the buffer concentration and the magnitude of the superimposed hydrodynamic flow on separation performance in CZE with contactless conductivity detection was carried out with capillaries of 10, 25, and 50 m internal diameter. It was confirmed that capillaries of narrow internal diameters require higher buffer concentrations for best sensitivities. For all diameters it was found that electrodispersion was the most pronounced band-broadening factor for relatively long residence times. For shorter times, Joule heating related band broadening appears to be the most significant factor, which means that best separation efficiencies are obtained with the narrowest capillaries. As detection limits are as good for capillaries of 10 m internal diameters as for the other diameters when using contactless conductivity detection, these narrow capillaries are, therefore, generally of benefit when employing this detection technique. Hydrodyamic flow was found to have only a very limited effect on band broadening; an effect was only noticeable for the 50 m capillary and relatively high flow rates.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
UniBasel Contributors:Hauser, Peter C. P. C.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Wiley-VCH
ISSN:0173-0835
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:31 Jan 2014 09:50
Deposited On:31 Jan 2014 09:50

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