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Determination of artificial sweeteners by capillary electrophoresis with contactless conductivity detection optimized by hydrodynamic pumping

Stojkovic, M. and Mai, T. D. and Hauser, P. C.. (2013) Determination of artificial sweeteners by capillary electrophoresis with contactless conductivity detection optimized by hydrodynamic pumping. Analytica chimica acta, Vol. 787. pp. 254-259.

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Official URL: http://edoc.unibas.ch/dok/A6212103

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Abstract

The common sweeteners aspartame, cyclamate, saccharin and acesulfame K were determined by capillary electrophoresis with contactless conductivity detection. In order to obtain the best compromise between separation efficiency and analysis time hydrodynamic pumping was imposed during the electrophoresis run employing a sequential injection manifold based on a syringe pump. Band broadening was avoided by using capillaries of a narrow 10 mu m internal diameter. The analyses were carried out in an aqueous running buffer consisting of 150 mM 2-(cyclohexylamino)ethanesulfonic acid and 400 mM tris(hydroxymethyl) aminomethane at pH 9.1 in order to render all analytes in the fully deprotonated anionic form. The use of surface modification to eliminate or reverse the electroosmotic flow was not necessary due to the superimposed bulk flow. The use of hydrodynamic pumping allowed easy optimization, either for fast separations (80 s) or low detection limits (6.5 mu mol L-1, 5.0 mu mol L-1, 4.0 mu mol L-1 and 3.8 mu mol L-1 for aspartame, cyclamate, saccharin and acesulfame K respectively, at a separation time of 190 s). The conditions for fast separations not only led to higher limits of detection but also to a narrower dynamic range. However, the settings can be changed readily between separations if needed. The four compounds were determined successfully in food samples. (C) 2013 Elsevier B.V. All rights reserved.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
UniBasel Contributors:Hauser, Peter C. P. C.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0003-2670
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:31 Jan 2014 09:50
Deposited On:31 Jan 2014 09:50

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