UV-absorbance detector for HPLC based on a light-emitting diode

Schmid, Stefan and Macka, Mirek and Hauser, Peter C.. (2008) UV-absorbance detector for HPLC based on a light-emitting diode. Analyst, 133 (4). pp. 465-469.

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Official URL: http://edoc.unibas.ch/dok/A6211750

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A flow-through optical absorption detector for HPLC was constructed using a novel deep-UV light-emitting diode as radiation source with a peak emission wavelength of 255 nm. For measuring the transmitted intensity (a property correlated to Transmittance) a special UV-sensitive photodiode was employed. Besides the power source, no optical or electronic components other than an inexpensive operational amplifier and a few passive components were necessary. The performance of the detector was tested with three substances, namely nitrobenzene, benzoic acid and methyl benzoate, which were separated by gradient elution using an acetonitrile/water mixture and tetrabutylammonium hydrogensulfate as pH-buffer. Calibration curves for concentrations between 1.6 mu g(.)mL(-1) and 400 mu g(.)mL(-1) (nitrobenzene) and 8 mu g(.)mL(-1) and 2.5 mg(.)mL(-1) (benzoic acid and methyl benzoate) were determined and coefficients of determination, r(2), of 0.9945, 0.9972 and 0.9996 were obtained for quadratic curve fits for the 3 compounds respectively. Relative standard deviations (n = 7) for peak areas were determined as 0.35 acid, 400 mu g(.)mL(-1)) and 0.83 g(.)mL(-1)). The lower limits of detection were found to be 750 ng(.)mL(-1), 5.8 mu g(.)mL(-1) and 12 mu g(.)mL(-1) for nitrobenzene, benzoic acid and methyl benzoate respectively.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
UniBasel Contributors:Hauser, Peter C. P. C.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Royal Society of Chemistry
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:29 Mar 2017 13:51
Deposited On:31 Jan 2014 09:49

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