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Development of polymorphic microsatellite markers for the asp viper (Vipera aspis) using high-throughput sequencing and their use for other European vipers

Geser, S. and Kaiser, L. and Zwahlen, V. and Ursenbacher, S.. (2013) Development of polymorphic microsatellite markers for the asp viper (Vipera aspis) using high-throughput sequencing and their use for other European vipers. Amphibia-Reptilia, Bd. 31, H. 1. pp. 109-113.

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Official URL: http://edoc.unibas.ch/dok/A6205235

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Abstract

The Asp viper (Vipera aspis) is a highly endangered species in the Swiss Plateau and in the Jura Mountains of Switzerland. In the past, numerous populations disappeared due to human activities, but currently the trend is reversed and several locations seem favourable for this species. However, it is not known if the remaining populations are still genetically variable and viable. In this study, 12 new microsatellite loci markers were developed for Vipera aspis using Next Generation Sequencing (NGS) techniques (454 sequencing). All markers proved to be polymorphic with 2-9 alleles. When cross-tested with two Eurasian widespread viper species, 8 of the newly developed loci provided reliable amplification for V. berus and 3 loci for V. ammodytes.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Natur- Landschafts- und Umweltschutz > Naturschutzbiologie (Baur)
UniBasel Contributors:Ursenbacher, Sylvain and Geser, Silvia and Kaiser, Laura and Zwahlen, Valerie
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Brill
ISSN:0173-5373
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:31 Jan 2014 09:49
Deposited On:31 Jan 2014 09:49

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