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Endothelialized tissue engineered 3D-construct for bone repair

Meury, Thomas. Endothelialized tissue engineered 3D-construct for bone repair. 2005, Doctoral Thesis, University of Basel, Faculty of Science.

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Official URL: http://edoc.unibas.ch/diss/DissB_7288

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Abstract

One of the main problems arising when implanting a graft into a large bone gap is its insufficient vascularization in the central region. This results in impaired bone healing and often leads to necrosis in the graft. Vascularization and bone formation have been shown to be very closely connected. In order to better understand the relationship between blood vessels and bone formation, we have studied the interactions between mesenchymal stem cells (MSC) and endothelial cells (EC). Since the blood vessel network undergoes dramatic remodeling during wound healing, a process that is connected with different endothelial cell maturational stages (reflected i.e. by different cell surface markers), we have also investigated the effect of growth factor-stimulated EC on MSC differentiation into bone forming cells. In addition, since during bone healing, one of the first events is the formation of a blood clot at the site of injury, we have evaluated the potential of platelet-rich plasma (PRP) as an osteoinductive substance by assessing its action on MSC differentiation. Finally, we combined all components into one autologous construct and to in vitro evaluate its potential to serve as a possible bone graft that could overcome the in vivo problem of limited vascularization generally occurring in large bone defects.
Advisors:Aebi, Ueli
Committee Members:Primig, Michael and Cancedda, Ranieri
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Aebi)
UniBasel Contributors:Aebi, Ueli
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:7288
Thesis status:Complete
Bibsysno:Link to catalogue
Number of Pages:147
Language:English
Identification Number:
Last Modified:22 Jan 2018 15:50
Deposited On:13 Feb 2009 15:17

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