The Pseudomonas type III effector HopQ1 activates cytokinin signaling and interferes with plant innate immunity

Hann, Dagmar R. and Domínguez-Ferreras, Ana and Motyka, Vaclav and Dobrev, Petre I. and Schornack, Sebastian and Jehle, Anna and Felix, Georg and Chinchilla, Delphine and Rathjen, John P. and Boller, Thomas. (2013) The Pseudomonas type III effector HopQ1 activates cytokinin signaling and interferes with plant innate immunity. New phytologist, Vol. 201, H. 2 , S. 585–598.

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Official URL: http://edoc.unibas.ch/dok/A6205464

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We characterized the molecular function of the Pseudomonas syringae pv. tomato DC3000 (Pto) effector HopQ1. In silico studies suggest that HopQ1 might possess nucleoside hydrolase activity based on the presence of a characteristic aspartate motif. Transgenic Arabidopsis lines expressing HopQ1 or HopQ1 aspartate mutant variants were characterized with respect to flagellin triggered immunity, phenotype and changes in phytohormone content by high-performance liquid chromatography-MS (HPLC-MS). We found that HopQ1, but not its aspartate mutants, suppressed all tested immunity marker assays. Suppression of immunity was the result of a lack of the flagellin receptor FLS2, whose gene expression was abolished by HopQ1 in a promoter-dependent manner. Furthermore, HopQ1 induced cytokinin signaling in Arabidopsis and the elevation in cytokinin signaling appears to be responsible for the attenuation of FLS2 expression. We conclude that HopQ1 can activate cytokinin signaling and that moderate activation of cytokinin signaling leads to suppression of FLS2 accumulation and thus defense signaling.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Ehemalige Einheiten Umweltwissenschaften > Pflanzenphysiologie Pathogenabwehr (Boller)
UniBasel Contributors:Boller, Thomas and Chinchilla, Delphine and Hann, Dagmar and Dominguez-Ferreras, Ana
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Blackwell Science
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Oct 2014 09:19
Deposited On:31 Jan 2014 09:49

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