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New microsatellite markers for Anthyllis vulneraria (Fabaceae), analyzed with Spreadex gel electrophoresis

Kesselring, Halil and Hamann, Elena and Stöcklin, Jürg and Armbruster, Georg F. J.. (2013) New microsatellite markers for Anthyllis vulneraria (Fabaceae), analyzed with Spreadex gel electrophoresis. Applications in Plant Sciences, 1 (12). p. 1300054.

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Official URL: http://edoc.unibas.ch/dok/A6205282

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Abstract

Premise of the study: New microsatellite primers were developed for the diploid herb Anthyllis vulneraria . These primers will be used in upcoming studies focusing on random genetic variation, local adaptation, and phenotypic plasticity in alpine plants. Methods and Results: The new primers were adjusted to separate PCR amplicons (70 to 170 bp) on precast Spreadex gels using horizontal gel electrophoresis. No capillary sequencer was needed. Three to twelve alleles were found per locus depending on the population studied. Conclusions: Our preliminary results showed that the three studied alpine populations are predominantly outcrossing, but include variable levels of self-fertilization.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Ehemalige Einheiten Umweltwissenschaften > Pflanzenökologie (Körner)
UniBasel Contributors:Armbruster, Georg FJ and Stöcklin, Jürg and Kesselring, Halil and Hamann, Elena
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Botanical Society of America
e-ISSN:2168-0450
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:13 Nov 2017 14:39
Deposited On:31 Jan 2014 09:49

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