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Structural constraints from residual tensorial couplings in high resolution NMR without an explicit term for the alignment tensor

Moltke, S. and Grzesiek, S.. (1999) Structural constraints from residual tensorial couplings in high resolution NMR without an explicit term for the alignment tensor. Journal of Biomolecular NMR, 15 (1). pp. 77-82.

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Official URL: http://edoc.unibas.ch/dok/A5258815

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Abstract

A simple, sensitivity-enhanced HSQC experiment is described which separates the upfield and downfield components in the indirect dimension into different subspectra. The sequence is similar to the generalized TROSY scheme; however, decoupling of the X-nucleus is used during detection. A detailed analysis of relaxation effects, precision and sensitivity of the method is presented. The approach is demonstrated in a two-dimensional water flip-back 1H-15N HSQC which measures 1JHN splittings in isotropic and oriented samples of ubiquitin and the hepatitis C protease. The results are in excellent agreement with splittings obtained from a conventional 1H-coupled HSQC.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Grzesiek)
UniBasel Contributors:Grzesiek, Stephan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Springer
ISSN:0925-2738
e-ISSN:1573-5001
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:23 Nov 2017 11:33
Deposited On:22 Mar 2012 13:21

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