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Solution NMR of proteins within polyacrylamide gels : diffusional properties and residual alignment by mechanical stress or embedding of oriented purple membranes

Sass, H. J. and Musco, G. and Stahl, S. J. and Wingfield, P. T. and Grzesiek, S.. (2000) Solution NMR of proteins within polyacrylamide gels : diffusional properties and residual alignment by mechanical stress or embedding of oriented purple membranes. Journal of Biomolecular NMR, 18 (4). pp. 303-309.

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Official URL: http://edoc.unibas.ch/dok/A5258807

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Abstract

The diffusive properties of biomacromolecules within the aqueous phase of polyacrylamide gels are described. High quality NMR spectra can be obtained under such conditions. As compared to water, a fivefold reduction in the translational diffusion constant, but only a 1.6-fold decrease (1.4-fold increase) in amide-15N T2 (T1) are observed for human ubiquitin within a 10% acrylamide gel. Weak alignment of the solute macromolecules can be achieved within such gels by vertical or radial compression or by the embedding of magnetically oriented purple membrane fragments. The methods are applied to deriveresidual dipolar couplings for human HIV-1 Nef and ubiquitin.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Grzesiek)
UniBasel Contributors:Grzesiek, Stephan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Springer
ISSN:0925-2738
e-ISSN:1573-5001
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:23 Nov 2017 11:29
Deposited On:22 Mar 2012 13:21

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