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Limiting factors in single particle cryo electron tomography

Kudryashev, Mikhail and Castaño-Díez, Daniel and Stahlberg, Henning. (2012) Limiting factors in single particle cryo electron tomography. Computational and structural biotechnology journal, Vol. 1, H. 1 , e201207002.

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Official URL: http://edoc.unibas.ch/dok/A6183906

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Abstract

Modern methods of cryo electron microscopy and tomography allow visualization of protein nanomachines in their native state at the nanometer scale. Image processing methods including sub-volume averaging applied to repeating macromolecular elements within tomograms allow exploring their structures within the native context of the cell, avoiding the need for protein isolation and purification. Today, many different data acquisition protocols and software solutions are available to researchers to determine average structures of macromolecular complexes and potentially to classify structural intermediates. Here, we list the density maps reported in the literature, and analyze each structure for the chosen instrumental settings, sample conditions, main processing steps, and obtained resolution. We present conclusions that identify factors currently limiting the resolution gained by this approach.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Research Network of Computational and Structural Biotechnology (RNCSB)
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:04 Sep 2015 14:32
Deposited On:06 Dec 2013 09:35

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