Structural analysis of B-Box 2 from MuRF1 : identification of a novel self-association pattern in a RING-like fold

Mrosek, M. and Meier, S. and Ucurum-Fotiadis, Z. and von Castelmur, E. and Hedbom, E. and Lustig, A. and Grzesiek, S. and Labeit, D. and Labeit, S. and Mayans, O.. (2008) Structural analysis of B-Box 2 from MuRF1 : identification of a novel self-association pattern in a RING-like fold. Biochemistry, Vol. 47, H. 40. pp. 10722-10730.

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Official URL: http://edoc.unibas.ch/dok/A5258763

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The B-box motif is the defining feature of the TRIM family of proteins, characterized by a RING finger-B-box-coiled coil tripartite fold. We have elucidated the crystal structure of B-box 2 (B2) from MuRF1, a TRIM protein that supports a wide variety of protein interactions in the sarcomere and regulates the trophic state of striated muscle tissue. MuRF1 B2 coordinates two zinc ions through a cross-brace alpha/beta-topology typical of members of the RING finger superfamily. However, it self-associates into dimers with high affinity. The dimerization pattern is mediated by the helical component of this fold and is unique among RING-like folds. This B2 reveals a long shallow groove that encircles the C-terminal metal binding site ZnII and appears as the defining protein-protein interaction feature of this domain. A cluster of conserved hydrophobic residues in this groove and, in particular, a highly conserved aromatic residue (Y133 in MuRF1 B2) is likely to be central to this role. We expect these findings to aid the future exploration of the cellular function and therapeutic potential of MuRF1.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Grzesiek)
05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Mayans)
UniBasel Contributors:Grzesiek, Stephan and Mayans, Olga
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Sep 2012 06:50
Deposited On:22 Mar 2012 13:20

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