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Band-selective ¹H-¹³C cross-polarization in fast magic angle spinning solid-state NMR spectroscopy

Laage, S. and Marchetti, A. and Sein, J. and Pierattelli, R. and Sass, H. J. and Grzesiek, S. and Lesage, A. and Pintacuda, G. and Emsley, L.. (2008) Band-selective ¹H-¹³C cross-polarization in fast magic angle spinning solid-state NMR spectroscopy. Journal of the American Chemical Society, Vol. 130, H. 51. pp. 17216-17217.

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Official URL: http://edoc.unibas.ch/dok/A5258758

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Abstract

A magic angle spinning (MAS) NMR technique to transfer polarization from protons to a specific set of the (13)C spins is introduced for the study of biomolecular samples in the solid-state. Ultrafast (<60 kHz) MAS and low irradiation rf fields are used to achieve band-selective Hartmann-Hahn cross-polarization (CP) between the whole proton bath and carbons whose resonances are close to the (13)C-transmitter offset. When compared to conventional, broadband (1)H-(13)C CP, the band-selective experiment can be established without any loss of sensitivity when polarizing the aliphatic signals of a protein sample, and with a significant gain when polarizing carbonyls. This scheme can be used as a building block in 2D (13)C-(13)C homonuclear correlation experiments to obtain a faster and more sensitive characterization of biological solids.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Grzesiek)
UniBasel Contributors:Grzesiek, Stephan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Chemical Society
ISSN:0002-7863
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:20
Deposited On:22 Mar 2012 13:20

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