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PICH: a DNA Translocase Specially Adapted for Processing Anaphase Bridge DNA

Biebricher, Andreas and Hirano, Seiki and Enzlin, Jacqueline H. and Wiechens, Nicola and Streicher, Werner W. and Huttner, Diana and Wang, Lily H.-C. and Nigg, Erich A. and Owen-Hughes, Tom and Liu, Ying and Peterman, Erwin and Wuite, Gijs J. L. and Hickson, Ian D.. (2013) PICH: a DNA Translocase Specially Adapted for Processing Anaphase Bridge DNA. Molecular cell, Vol. 51, H. 5. pp. 691-701.

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Official URL: http://edoc.unibas.ch/dok/A6174405

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Abstract

The Plk1-interacting checkpoint helicase (PICH) protein localizes to ultrafine anaphase bridges (UFBs) in mitosis alongside a complex of DNA repair proteins, including the Bloom's syndrome protein (BLM). However, very little is known about the function of PICH or how it is recruited to UFBs. Using a combination of microfluidics, fluorescence microscopy, and optical tweezers, we have defined the properties of PICH in an in vitro model of an anaphase bridge. We show that PICH binds with a remarkably high affinity to duplex DNA, resulting in ATP-dependent protein translocation and extension of the DNA. Most strikingly, the affinity of PICH for binding DNA increases with tension-induced DNA stretching, which mimics the effect of the mitotic spindle on a UFB. PICH binding also appears to diminish force-induced DNA melting. We propose a model in which PICH recognizes and stabilizes DNA under tension during anaphase, thereby facilitating the resolution of entangled sister chromatids.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg)
UniBasel Contributors:Nigg, Erich A.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Cell Press
ISSN:1097-2765
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:25 Oct 2013 08:33
Deposited On:25 Oct 2013 08:33

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