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Molecular basis of tubulin transport within the cilium by IFT74 and IFT81

Bhogaraju, Sagar and Cajanek, Lukas and Fort, Cécile and Blisnick, Thierry and Weber, Kristina and Taschner, Michael and Mizuno, Naoko and Lamla, Stefan and Bastin, Philippe and Nigg, Erich A. and Lorentzen, Esben. (2013) Molecular basis of tubulin transport within the cilium by IFT74 and IFT81. Science, Vol. 341, H. 6149. pp. 1009-1012.

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Official URL: http://edoc.unibas.ch/dok/A6174406

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Abstract

Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg)
UniBasel Contributors:Nigg, Erich A.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Association for the Advancement of Science
ISSN:0036-8075
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:25 Oct 2013 08:33
Deposited On:25 Oct 2013 08:33

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