edoc

Human Cep192 and Cep152 cooperate in Plk4 recruitment and centriole duplication

Sonnen, Katharina F. and Gabryjonczyk, Anna-Maria and Anselm, Eduard and Stierhof, York-Dieter and Nigg, Erich A.. (2013) Human Cep192 and Cep152 cooperate in Plk4 recruitment and centriole duplication. Journal of cell science, Vol. 126, H. 14. pp. 3223-3233.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A6124651

Downloads: Statistics Overview

Abstract

Polo-like kinase 4 (Plk4) is a key regulator of centriole duplication, but the mechanism underlying its recruitment to mammalian centrioles is not understood. In flies, Plk4 recruitment depends on Asterless, whereas nematodes rely on a distinct protein, Spd-2. Here, we have explored the roles of two homologous mammalian proteins, Cep152 and Cep192, respectively, in the centriole recruitment of human Plk4. We demonstrate that Cep192 plays a key role in centrosome recruitment of both Cep152 and Plk4. Double-depletion of Cep192 and Cep152 completely abolishes Plk4 binding to centrioles as well as centriole duplication, indicating that the two proteins cooperate. Most importantly, we show that Cep192 binds Plk4 through an N-terminal extension that is specific to the largest isoform. The Plk4 binding regions of Cep192 and Cep152 (residues 190-240 and 1-46, respectively) are rich in negatively charged amino acids, suggesting that Plk4 localization to centrioles depends on electrostatic interactions with the positively charged polo-box domain. We conclude that cooperation between Cep192 and Cep152 is crucial for centriole recruitment of Plk4 and centriole duplication during the cell cycle.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg)
UniBasel Contributors:Nigg, Erich A.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Company of Biologists
ISSN:0021-9533
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:25 Oct 2013 08:33
Deposited On:25 Oct 2013 08:33

Repository Staff Only: item control page