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Plasmodium falciparum centromeres display a unique epigenetic makeup and cluster prior to and during schizogony

Hoeijmakers, W. A. and Flueck, C. and Françoijs, K. J. and Smits, A. H. and Wetzel, J. and Volz, J. C. and Cowman, A. F. and Voss, Till S. and Stunnenberg, H. G. and Bártfai, R.. (2012) Plasmodium falciparum centromeres display a unique epigenetic makeup and cluster prior to and during schizogony. Cellular microbiology, Vol. 14, H. 9. pp. 1391-1401.

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Official URL: http://edoc.unibas.ch/dok/A6094316

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Abstract

Centromeres are essential for the faithful transmission of chromosomes to the next generation, therefore being essential in all eukaryotic organisms. The centromeres of Plasmodium falciparum, the causative agent of the most severe form of malaria have been broadly mapped on most chromosomes, but their epigenetic composition remained undefined. Here, we reveal that the centromeric histone variant PfCENH3 occupies a 4-4.5kb region on each P. falciparum chromosome, which is devoid of pericentric heterochromatin but harbors another histone variant, PfH2A.Z. These CENH3 covered regions pinpoint the exact position of the centromere on all chromosomes and revealed that all centromeric regions have similar size and sequence composition. Immunofluorescence assay of PfCENH3 strongly suggests that P. falciparum centromeres cluster to a single nuclear location prior to and during mitosis and cytokinesis but dissociate soon after invasion. In summary, we reveal a dynamic association of Plasmodium centromeres, which bear a unique epigenetic signature and conform to a strict structure. These findings suggest that DNA-associated and epigenetic elements play an important role in centromere establishment in this important human pathogen.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Gene Regulation (Voss)
UniBasel Contributors:Voss, Till S and Flück, Christian
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Blackwell
ISSN:1462-5814
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:06 Nov 2018 13:42
Deposited On:16 Aug 2013 07:29

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