Chicken egg yolk antibodies (IgY) for detecting circulating antigens of Schistosoma japonicum

Cai, Yu-Chun and Guo, Jian and Chen, Shao-Hong and Tian, Li-Guang and Steinmann, Peter and Chen, Mu-Xin and Li, Hao and Ai, Lin and Chen, Jia-Xu. (2012) Chicken egg yolk antibodies (IgY) for detecting circulating antigens of Schistosoma japonicum. Parasitology international, Vol. 61, H. 3. pp. 385-390.

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Official URL: http://edoc.unibas.ch/dok/A6094093

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BACKGROUND: IgY isolated from egg yolk has been widely used in immunodiagnostic tests, including tests to detect circulating antigen (soluble egg antigen or SEA) of Schistosoma japonicum. RESULTS: A sandwich ELISA was established using a combination of anti-S. japonicum SEA-IgY polyclonal antibodies and IgM monoclonal antibodies. To explore sensitivity and specificity of the sandwich ELISA, serum samples from 43 patients infected with S. japonicum were tested. All acute cases and 91.3% of the chronic cases showed a positive reaction. Only 5% of the control sera from healthy persons gave a positive response. Cross-reactions with antibodies to nine other parasites were rare. CONCLUSION: The developed immunoassay is reasonably sensitive and specific. It could be used for field research and treatment efficacy assessments
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Eco System Health Sciences
UniBasel Contributors:Steinmann, Peter
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:04 Sep 2015 14:31
Deposited On:19 Jul 2013 07:43

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