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Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (Oo)cysts using transportable instrumentation in a field deployment

Keserue, H. A. and Füchslin, H. P. and Wittwer, M. and Nguyen-Viet, H. and Nguyen, T. T. and Surkinkul, N. and Koottatep, T. and Schürch, N. and Egli, T.. (2012) Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (Oo)cysts using transportable instrumentation in a field deployment. Environmental science & technology, Vol. 46, H. 16. pp. 8952-8959.

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Official URL: http://edoc.unibas.ch/dok/A6094110

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Abstract

Reliable, sensitive, quantitative, and mobile rapid screening methods for pathogenic organisms are not yet readily available, but would provide a great benefit to humanitarian intervention units in disaster situations. We compared three different methods (immunofluorescent microscopy, IFM; flow cytometry, FCM; polymerase chain reaction, PCR) for the rapid and quantitative detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in a field campaign. For this we deployed our mobile instrumentation and sampled canal water and vegetables during a 2 week field study in Thailand. For purification and concentrations of (oo)cysts, we used filtration and immunomagnetic separation. We were able to detect considerably high oo(cysts) concentrations (ranges: 15-855 and 0-240 oo(cysts)/liter for Giardia and Cryptosporidium, respectively) in 85 to 300 min, with FCM being fastest, followed by PCR, and IFM being slowest due to the long analysis time per sample. FCM and IFM performed consistently well, whereas PCR reactions often failed. The recovery, established by FCM, was around 30% for Giardia and 13% for Cryptosporidium (oo)cysts. It was possible to track (oo)cysts from the wastewater further downstream to irrigation waters and confirm contamination of salads and water vegetables. We believe that rapid detection, in particular FCM-based methods, can substantially help in disaster management and outbreak prevention.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Human and Animal Health
UniBasel Contributors:Nguyen Viet, Hung
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Chemical Soc.
ISSN:0013-936X
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:19 Jul 2013 07:44
Deposited On:19 Jul 2013 07:42

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