Short communication: appropriate and alternative methods to determine viable bacterial counts in cow milk samples

Loss, G. and Apprich, S. and Kneifel, W. and von Mutius, E. and Genuneit, J. and Braun-Fahrländer, C.. (2012) Short communication: appropriate and alternative methods to determine viable bacterial counts in cow milk samples. Journal of dairy science, Vol. 95, H. 6. pp. 2916-2918.

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Official URL: http://edoc.unibas.ch/dok/A6146281

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Farm milk consumption is reported to be inversely related to the development of asthma and atopy in children and it has been hypothesized that microorganisms in milk might contribute to this protective effect. The GABRIEL study was designed to investigate this hypothesis in a large population of European children, calling for a rapid alternative to classical culture techniques to determine bacteriological properties of milk samples. One objective was to evaluate 2 different rapid methods to determine bacteriological properties in a large number of cow milk samples collected under field conditions. BactoScan (Foss Analytical, Hillerod, Denmark), an automated standard flow cytometric method utilized for routine testing of milk quality, and TEMPO (bioMerieux, Marcy l'Etoile, France), an automated most-probable-number method, were used to assess the total viable bacterial count in farm and commercial milk samples. Both methods were compared with standard plate count method and each other. Measurements based on the TEMPO method were in good agreement with the standard plate count method and showed reliable results, whereas BactoScan results did not correlate with standard plate count measurements and yielded higher bacteria counts in heat-treated milk samples compared with raw milk samples. Most likely, these discrepant results were due to inferences with staining reactions and detection of bacteria in heat-treated milk samples. We conclude that, in contrast to the routinely used BactoScan method, the TEMPO method is an inexpensive and rapid alternative to standard culture methods suitable to assess total bacterial counts in processed and raw milk samples
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Former Units within Swiss TPH > Microbial Exposure & Childhood Allergies (Braun-Fahrländer)
UniBasel Contributors:Loss, Georg and Braun-Fahrländer, Charlotte
Item Type:Article, refereed
Article Subtype:Book Review
Publisher:American Dairy Science Association
Note:Publication type according to Uni Basel Research Database: Journal item
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Last Modified:19 Jul 2013 07:43
Deposited On:19 Jul 2013 07:39

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