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Dual RMCE for efficient re-engineering of mouse mutant alleles

Osterwalder, Marco and Galli, Antonella and Rosen, Barry and Skarnes, William C. and Zeller, Rolf and Lopez-Rios, Javier. (2010) Dual RMCE for efficient re-engineering of mouse mutant alleles. Nature methods, Vol. 7, H. 11. pp. 893-895.

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Official URL: http://edoc.unibas.ch/dok/A6004287

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Abstract

We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Division of Anatomy > Developmental Genetics (Zeller/Zuniga)
UniBasel Contributors:Zeller, Rolf
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Nature Publishing Group
ISSN:1548-7091
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:19 Jul 2013 07:43
Deposited On:19 Jul 2013 07:35

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