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Essential role for cholesterol in the delivery of exogenous antigens to the MHC class I-presentation pathway

Albrecht, I. and Gatfield, J. and Mini, T. and Jeno, P. and Pieters, J.. (2006) Essential role for cholesterol in the delivery of exogenous antigens to the MHC class I-presentation pathway. International immunology, 18 (5). pp. 755-765.

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Official URL: http://edoc.unibas.ch/dok/A5258303

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Abstract

Cross-presentation, which is crucial for the generation of immunity against virus-infected and tumor cells, requires exogenous antigens to be internalized into antigen-presenting cells (APCs) followed by translocation to the cytosol by unknown mechanisms. One important entry route for such antigens is macropinocytosis. We here describe that cholesterol is essential for cross-presentation of antigens loaded via macropinocytosis into APCs. Modification of antigens by palmitoylation to target antigens to cholesterol-enriched plasma membrane domains resulted in a dramatically increased T cell activation. These results define cholesterol as an essential factor for cross-presentation and suggest that specific modification of antigens to increase their affinity for cholesterol may be utilized to enhance immunity.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Biochemistry (Pieters)
05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Mass Spectrometry (Jenö)
UniBasel Contributors:Jenö, Paul and Pieters, Jean
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Oxford University Press
ISSN:0953-8178
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:25 Sep 2017 14:05
Deposited On:22 Mar 2012 13:20

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