edoc

Structural and functional alterations of a colicin-resistant mutant of OmpF porin from Escherichia coli

Jeanteur, D. and Schirmer, T. and Fourel, D. and Simonet, V. and Rummel, G. and Widmer, C. and Rosenbusch, J. P. and Pattus, F. and Pagès, J. M.. (1994) Structural and functional alterations of a colicin-resistant mutant of OmpF porin from Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, H. 22. pp. 10675-10679.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5258287

Downloads: Statistics Overview

Abstract

A strain of Escherichia coli, selected on the basis of its resistance to colicin N, reveals distinct structural and functional alterations in unspecific OmpF porin. A single mutation [Gly-119--<Asp (G119D)] was identified in the internal loop L3 that contributes critically to the formation of the construction inside the lumen of the pore. X-ray structure analysis to a resolution of 3.0 A reveals a locally altered peptide backbone, with the side chain of residue Asp-119 protruding into the channel, causing the area of the constriction (7 x 11 A in the wild type) to be subdivided into two intercommunicating subcompartments of 3-4 A in diameter. The functional consequences of this structural modification consist of a reduction of the channel conductance by about one-third, of altered ion selectivity and voltage gating, and of a decrease of permeation rates of various sugars by factors of 2-12. The structural modification of the mutant protein affects neither the beta-barrel structure nor those regions of the molecule that are exposed at the cell surface. Considering the colicin resistance of the mutant, it is inferred that in vivo, colicin N traverses the outer membrane through the porin channel or that the dynamics of the exposed loops are affected in the mutant such that these may impede the binding of the toxin.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Schirmer)
UniBasel Contributors:Schirmer, Tilman
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:National Academy of Sciences
ISSN:0027-8424
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:07 Aug 2015 12:05
Deposited On:22 Mar 2012 13:20

Repository Staff Only: item control page