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Orderly osteochondral regeneration in a sheep model using a novel nano-composite multilayered biomaterial

Kon, Elizaveta and Delcogliano, Marco and Filardo, Giuseppe and Fini, Milena and Giavaresi, Gianluca and Francioli, Silvia and Martin, Ivan and Pressato, Daniele and Arcangeli, Elena and Quarto, Rodolfo and Sandri, Monica and Marcacci, Maurillo. (2010) Orderly osteochondral regeneration in a sheep model using a novel nano-composite multilayered biomaterial. Journal of orthopaedic research, Vol. 28, H. 1. pp. 116-124.

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Official URL: http://edoc.unibas.ch/dok/A6005279

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Abstract

The objective of this article was to investigate the safety and regenerative potential of a newly developed biomimetic scaffold when applied to osteochondral defects in an animal model. A new multilayer gradient nano-composite scaffold was obtained by nucleating collagen fibrils with hydroxyapatite nanoparticles. In the femoral condyles of 12 sheep, 24 osteochondral lesions were created. Animals were randomized into three treatment groups: scaffold alone, scaffold colonized in vitro with autologous chondrocytes and empty defects. Six months after surgery, the animals were sacrificed and the lesions were histologically evaluated. Histologic and gross evaluation of specimens showed good integration of the chondral surface in all groups except for the control group. Significantly better bone regeneration was observed both in the group receiving the scaffold alone and in the group with scaffold loaded with autologous chondrocytes. No difference in cartilage surface reconstruction and osteochondral defect filling was noted between cell-seeded and cell-free groups. In the control group, no bone or cartilage defect healing occurred, and the defects were filled with fibrous tissue. Quantitative macroscopic and histological score evaluations confirmed the qualitative trends observed. The results of the present study showed that this novel osteochondral scaffold is safe and easy to use, and may represent a suitable matrix to direct and coordinate the process of bone and hyaline-like cartilage regeneration. The comparable regeneration process observed with or without autologous chondrocytes suggests that the main mode of action of the scaffold is based on the recruitment of local cells.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:John Wiley
ISSN:0736-0266
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:24 May 2013 09:13
Deposited On:26 Apr 2013 07:01

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