Improvement of tendon repair using muscle grafts transduced with TGF-?1 cDNA

Majewski, M. and Porter, R. M. and Betz, O. B. and Betz, V. M. and Clahsen, H. and Flückiger, R. and Evans, C. H.. (2012) Improvement of tendon repair using muscle grafts transduced with TGF-?1 cDNA. European cells & materials, Vol. 23. pp. 94-102.

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Official URL: http://edoc.unibas.ch/dok/A6006538

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Tendon rupture is a common injury. Inadequate endogenous repair often leaves patients symptomatic, with tendons susceptible to re-rupture. Administration of certain growth factors improves tendon healing in animal models, but their delivery remains a challenge. Here we evaluated the delivery of TGF-?1 to tendon defects by the implantation of genetically modified muscle grafts. Rat muscle biopsies were transduced with recombinant adenovirus encoding TGF-?1 and grafted onto surgically transected Achilles tendons in recipient animals. Tissue regenerates were compared to those of controls by biomechanical testing as well as histochemical and immunohistochemical analyses. Healing was greatly accelerated when genetically modified grafts were implanted into tendon defects, with the resulting repair tissue gaining nearly normal histological appearance as early as 2 weeks postoperatively. This was associated with decreased deposition of type III collagen in favour of large fibre bundles indicative of type I collagen. These differences in tendon composition coincided with accelerated restoration of mechanical strength. Tendon thickness increased in gene-treated animals at weeks 1 and 2, but by week 8 became significantly lower than that of controls suggesting accelerated remodelling. Thus localised TGF-?1 delivery via adenovirus-modified muscle grafts improved tendon healing in this rat model and holds promise for clinical application.
Faculties and Departments:03 Faculty of Medicine > Bereich Operative Fächer (Klinik) > Bewegungsapparat und Integument
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Operative Fächer (Klinik) > Bewegungsapparat und Integument
UniBasel Contributors:Majewski, Martin
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Univ. of Wales
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:24 May 2013 09:14
Deposited On:26 Apr 2013 06:59

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