Lack of mismatch correction facilitates genome evolution in mycobacteria

Springer, Burkhard and Sander, Peter and Sedlacek, Ludwig and Hardt, Wolf-Dietrich and Mizrahi, Valerie and Schär, Primo and Böttger, Erik C.. (2004) Lack of mismatch correction facilitates genome evolution in mycobacteria. Molecular Microbiology, Vol. 53, H. 6. pp. 1601-1609.

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Official URL: http://edoc.unibas.ch/dok/A5839355

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In silico genome sequence analyses suggested that mycobacteria are devoid of the highly conserved mutLS-based post-replicative mismatch repair system. Here, we present the first biological evidence for the lack of a classical mismatch repair function in mycobacteria. We found that frameshifts, but not general mutation rates are unusually high in Mycobacterium smegmatis. However, despite the absence of mismatch correction, M. smegmatis establishes a strong barrier to recombination between homeologous DNA sequences. We show that 10-12% of DNA sequence heterology restricts initiation of recombination but not extension of heteroduplex DNA intermediates. Together, the lack of mismatch correction and a high stringency of initiation of homologous recombination provide an adequate strategy for mycobacterial genome evolution, which occurs by gene duplication and divergent evolution.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Division of Biochemistry and Genetics > Molecular Genetics (Schär)
UniBasel Contributors:Schär, Primo Leo
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:24 May 2013 09:10
Deposited On:26 Apr 2013 06:57

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