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A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis

Picotti, Paola and Clément-Ziza, Mathieu and Lam, Henry and Campbell, David S. and Schmidt, Alexander and Deutsch, Eric W. and Röst, Hannes and Sun, Zhi and Rinner, Oliver and Reiter, Lukas and Shen, Qin and Michaelson, Jacob J. and Frei, Andreas and Alberti, Simon and Kusebauch, Ulrike and Wollscheid, Bernd and Moritz, Robert L. and Beyer, Andreas and Aebersold, Ruedi. (2013) A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis. Nature, Vol. 494, H. 7436. pp. 266-270.

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Official URL: http://edoc.unibas.ch/dok/A6094005

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Abstract

Experience from different fields of life sciences suggests that accessible, complete reference maps of the components of the system under study are highly beneficial research tools. Examples of such maps include libraries of the spectroscopic properties of molecules, or databases of drug structures in analytical or forensic chemistry. Such maps, and methods to navigate them, constitute reliable assays to probe any sample for the presence and amount of molecules contained in the map. So far, attempts to generate such maps for any proteome have failed to reach complete proteome coverage. Here we use a strategy based on high-throughput peptide synthesis and mass spectrometry to generate an almost complete reference map (97% of the genome-predicted proteins) of the Saccharomyces cerevisiae proteome. We generated two versions of this mass-spectrometric map, one supporting discovery-driven (shotgun) and the other supporting hypothesis-driven (targeted) proteomic measurements. Together, the two versions of the map constitute a complete set of proteomic assays to support most studies performed with contemporary proteomic technologies. To show the utility of the maps, we applied them to a protein quantitative trait locus (QTL) analysis, which requires precise measurement of the same set of peptides over a large number of samples. Protein measurements over 78 S. cerevisiae strains revealed a complex relationship between independent genetic loci, influencing the levels of related proteins. Our results suggest that selective pressure favours the acquisition of sets of polymorphisms that adapt protein levels but also maintain the stoichiometry of functionally related pathway members.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Proteomics (Schmidt)
UniBasel Contributors:Schmidt, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Macmillan
ISSN:0028-0836
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:24 May 2013 09:17
Deposited On:26 Apr 2013 06:54

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