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Characterization of purified DNA-relaxing enzyme from human tissue culture cells

Keller, W.. (1975) Characterization of purified DNA-relaxing enzyme from human tissue culture cells. Proceedings of the National Academy of Sciences of the United States of America, 72 (7). pp. 2550-2554.

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Official URL: http://edoc.unibas.ch/dok/A5258015

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Abstract

Superhelical simian virus 40 DNA migrates more rapidly during electrophoresis in agarose gels than covalently closed DNA free of superhelical turns (relaxed DNA). The difference in electrophoretic mobility between superhelical and relaxed DNA was used to monitor the activity of a protein from human tissue culture cells which converts superhelical DNA into relaxed DNA. Purified DNA-relaxing protein removes both negative and positive superhelical turns and acts in a catalytic manner. The relaxation of DNA proceeds in a stepwise fashion and DNA intermediates with decreasing numbers of superhelical turns are seen during the course of the reaction.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Keller)
UniBasel Contributors:Keller, Walter
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:National Academy of Sciences
ISSN:0027-8424
e-ISSN:1091-6490
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:07 Nov 2017 13:25
Deposited On:22 Mar 2012 13:19

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