High-Density Reconstitution of Functional Water Channels into Vesicular and Planar Block Copolymer Membranes

Kumar, M. and Habel, J. E. O. and Shen, Y. X. and Meier, W. P. and Walz, T.. (2012) High-Density Reconstitution of Functional Water Channels into Vesicular and Planar Block Copolymer Membranes. Journal of the American Chemical Society, 134 (45). pp. 18631-18637.

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Official URL: http://edoc.unibas.ch/dok/A6070459

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The exquisite selectivity and unique transport properties of membrane proteins can be harnessed for a variety of engineering and biomedical applications if suitable membranes can be produced Amphiphilic block copolymers (BCPs), developed as stable lipid analogs, form membranes that functionally incorporate membrane proteins and are ideal for such applications While high protein density and planar membrane morphology are most desirable, BCP-membrane, protein aggregates have so far been,limited to law protein densities in either vesicular or bilayer morphologies. Here, we used dialysis to reproducibly form planar and vesicular BCP membranes with a high density of reconstituted aquaporin-0 (AQP0) water channels. We show that AQP0 retains its biological activity when incorporated at high density in BCP membranes, and that the morphology of the BCP protein aggregates can be controlled by adjusting the amount of incorporated AQP0. We also show that BCPs can be used to form two-dimensional crystals of AQP0.
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:04 Jun 2018 12:29
Deposited On:01 Mar 2013 11:12

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